Clinical characterisation of women with persistent genital arousal disorder: the iPGAD-study

Persistent Genital Arousal Disorder (PGAD) is a rare condition—mostly in women—where patients perceive prolonged genital arousal without any sexual desire or stimulation. Etiopathological considerations reach from peripheral to central issues over local disturbance of the pudendal nerve to neuropathy, psychosocial, and pharmacological theories. Since well controlled clinical studies about PGAD in conjunction with a mental and somatic health status are missing, this study is a detailed clinical investigation of PGAD patients compared to healthy controls. 26 women who fulfilled diagnostic criteria for PGAD were compared to 26 age matched healthy controls. Investigations included comparison of vegetative, gynaecological and sexual history, psychiatric features as well as a (neuro-)radiological, neurophysiological and gynaecological examination. Moreover, a detailed clinical characterisation of PGAD symptoms was performed. PGAD symptoms were mostly characterised as tingling or prickling and were permanently present. In over 80%, PGAD symptoms were located in the clitoris. Almost 70% reported radiations to other regions of the body. Most frequent trigger factors were tight clothes, mental stress, driving a car/bus/bicycle and sexual intercourse. Relieving factors were mainly distraction, relaxation, physical exercise, masturbation and swimming. In group comparisons, PGAD presented with significant higher rates of sexual dysfunctions, spontaneous orgasms, swelling of the genitals, extraordinary lubrication as well as higher rates in depression, agoraphobia, generalized anxiety disorder and lifetime panic disorder. Significantly more PGAD patients were diagnosed with restless legs symptoms. In contrast childhood traumatization, somatization disorder, suicidality, gynaecological as well as neurophysiological examination of the pudendal nerve were not different between the groups. MRI of the brain, pelvis and spinal cord was unsuspicious and incidental findings - including Tarlov cysts or pelvic venous congestion - were equally distributed among the groups. In summary, our study provides a careful characterization of women with PGAD highlighting a serious mental burden, most probably as a consequence of PGAD. With the current set of clinical investigations there was no evidence of a clear causal relationship to a specific clinical finding as it has been previously discussed. Future studies and additional techniques will have to further explore where and how in the peripheral or central nervous systems PGAD develops.

There are some theories on the causes of PGAD pointing towards disturbances of the peripheral and/or central nervous system.Regarding peripheral factors compression of the dorsal branch of the pudendal nerve, e.g. by a periclitoral mass 10,12,15 , dorsal nerve injury due to pelvic trauma by e.g.horseback riding accidents 16 , bicycle riding 17 , birth injury or surgical trauma 18 are discussed.The pudendal nerve not only transmits the perception of stimulation from the clitoris with the branch of the dorsal clitoral nerve, which is then interpreted as a sensation of arousal, but also transmits the perceptions of the areas of the perineal and posterior labial nerves 19 .Hence, nerve compression or injury shall produce the symptoms of continuous arousal 18 .Small fibre neuropathy has also been discussed as a cause of PGAD and the frequent intolerance towards tight clothing and prolonged sitting as a trigger of PGAD may support this assumption 18,20,21 .However, respective (histo-)pathological findings have not yet been reported 6,12,22 .Oaklander et al. 23 suspect a connection to sensory polyneuropathy.Other theories suggest an overlap between PGAD, Restless Legs Syndrome (RLS) and Overactive Bladder Syndrome (OAB) 20,24 and assume a dysregulation of neurovegetative afferent and efferent signals, supposing PGAD as a phenotypic variant of RLS and OAB representing the same pathologic state only in different areas [25][26][27] .Contradicting this assumption reports on sustained symptom release by administration of dopaminergic or anticholinergic drugs are missing.From a still peripheral, vascular position, pelvic varices or pelvic congestion syndrome are discussed factors associated with PGAD 20,22 .
With regard to the central nervous system, sacral meningeal cysts (e.g.Tarlov cysts) 8,12,20 and intervertebral disc pathologies are discussed as possible causes of PGAD 8 .Other discussed neurological pathologies include epileptic foci 28 , sexual epileptic auras 29 , arteriovenous malformation 30 and arteriovenous fistulas and strokes 31 .On a neuropsychopharmacological level there is some evidence that antidepressants, antipsychotics and anticonvulsants can both induce symptoms of PGAD during administration or (probably more often) discontinuation of the drug or even alleviate symptoms 32,33 .Theories have been proposed that improvement may be due to inhibition of sexual perception and induction of symptoms by disinhibition induced by drugs with a primarily serotonergic function [34][35][36][37][38] .
In summary, many assumptions on possible causes and triggering factors of PGAD have been made, however, there is a lack of well controlled clinical studies (case control studies) incorporating a thorough clinical assessment of subjects with PGAD compared to age matched healthy controls.Researchers, clinicians and patients agree, that PGAD is a severely distressing and life-impairing disease, which requires intensified research based on systematic, controlled trials 8 .Based on previous research and above-mentioned possible causes of PGAD, Goldstein et al. 8 proposed a systematic investigation of PGAD patients.The investigation should include the following 5 regions: (1) end organ, (2) pelvis and perineum, (3) cauda equina, (4) spinal cord, (5) brain.In addition, a detailed history should be taken, including the exact symptomatology, trigger and relieving factors, mental health and medication history.Hence, we initiated a clinical investigation aiming at systematically elucidating the different clinical levels and components that might contribute to PGAD using a well-controlled comprehensive multimodal assessment of PGAD patients and controls.Goldstein et al. 8 also used the term of genito-pelvic dysesthesia (GPD).In this manuscript the consistently use of the term PGAD was agreed.

Subjects and procedure
A total of 52 subjects participated in the iPGAD study (Identification of Etiopathological and Clinical Factors in Persistent Genital Arousal Disorder: the iPGAD-Study), 26 patients suffering from PGAD and 26 control subjects.Data acquisition took place from June 2020 to August 2021.Subjects gave written informed consent to participate, were free to withdraw from the study at any time and received reimbursement for their participations.No subjects were excluded from the analyses (see Figs. 1 And 2).Patients suffering from PGAD were recruited via the sexual medicine consultation at Hannover Medical School or via social networks (in particular www.faceb ook.com and German internet forums for PGAD).The control subjects were recruited via the intranet of Hannover Medical School, social networks, and word of mouth.Control subjects were matched for age and years of education.This study was conducted in accordance with the Declaration of Helsinki 1964, updated in October 2013 and was approved by the Ethics Committee of Hannover Medical School (No. 8589_BO_S_2019).The study was registered at ClinicalTrials.gov(Protocol ID: 8589_BO_S_2019; NCT04566783).
Inclusion criteria for control subjects were defined as follows: female gender, age between 18 and 75 years, proficiency in German writing and language, no acute and severe mental or somatic disease requiring immediate treatment.In addition, PGAD patients should fulfil the criteria of Leiblum and Nathan 47 .These include: (1) Prolonged persistence of involuntary genital and clitoral arousal over an extended period of time (hours, days, or months), (2) no subsiding of genital arousal despite one or more orgasms, (3) no association of physical genital arousal with subjective feelings of sexual arousal or sexual desire, (4) the arousal is not only triggered by sexual activity, but also by non-sexual stimuli, or without any obvious triggers, (5) the arousal is experienced as unwanted and intrusive; correspondingly, distress is associated with it.A severe intelligence impairment, acute physical or mental illness (e.g.acute psychosis, brain damage, Alzheimer's disease, severe bacterial infection) and contraindication to magnetic resonance imaging were the exclusion criteria for both groups.

Sociodemographic data and neurodevelopmental factors
In a semi-structured interview, sociodemographic data including age, highest educational qualification converted into years of education, employment status (employed, in training, unemployed, retired), body size and weight (Body Mass Index), handedness (left-or right-handed), the use or abuse of alcohol, nicotine and recreational drugs, medication, marital status (unmarried or single, married, divorced) and the number of births, miscarriages and abortions were collected.

Gynaecology and vegetative history
In the context of a semi-structured interview, a survey of the gynaecological history including micturition (especially dysuria, urinary urgency and frequency), the duration of the menstrual cycle (excluding those who were menopausal or stopped menstruating due to hormonal contraception), dysmenorrhea or eumenorrhea, menopause, menarche, masturbarche, contraception and infertility treatment was taken.

Sexual history
The sexual orientation was accessed based on the Kinsey Scale 48 .The categories were modified to heterosexual, bisexual and homosexual.Hypersexual disorder was assessed using the four Kafka criteria 49 .Furthermore, the subjects were specifically asked for spontaneous orgasms, swelling of the genitals and an extraordinary lubrication.

Characterization of PGAD
In addition, the detailed history of the PGAD symptomatology was taken.The age at first symptomatology and the duration of the disease were assessed.PGAD patients were asked for trigger and relief factors and their medication concerning the PGAD symptoms.To better map the PGAD symptomatology, a specific questionnaire was developed based on the pain detect questionnaire 50 .Symptom severity was assessed at three different time points (now, most severe symptoms in the last 4 weeks, average symptom severity in the last 4 weeks) using visual analogue scale (VAS) from 0 to 10.The variables surveyed also included the course of the symptoms (continuous symptoms with slight fluctuations, continuous symptoms with symptom attacks, symptom attacks and symptom-free phases in between, symptom attacks and symptoms in between).The quality of the symptoms (burning, tingling or prickling, touch, lightning-like or electrifying, cold, heat/warmth, numbness, slight pressure) was measured by Likert-type questions (never, hardly, slight, moderate, strong, very strong).It was also assessed, whether there is radiation to other regions of the body (e.g., abdomen, back, legs, arms, breasts).To specify the exact localisation of the symptomatology, the patient could mark the concerned regions on a body illustration.They could set as many crosses as they wanted (mons pubis, urethra, vaginal opening, pubic bone, clitoris, inner and outer labia, anus, uterine region, ovarian region).

Psychological features including psychiatric comorbidities and personality
In a semi-structured interview, it was assessed whether ICD-10 criteria for sexual dysfunction (ICD10 F52, including lack or loss of sexual desire, sexual aversion, lack of sexual satisfaction, failure of genital responses, orgasmic disturbances, nonorganic vaginismus, non-organic dyspareunia and increased sexual desire) and somatization disorder (ICD-10 F45, including undifferentiated somatization disorder, hypochondriacal disorder, somatoform autonomic dysfunction, persistent somatoform pain disorder, other and unspecified somatoform disorder) were met.A possible somatoform pain disorder was measured by using the German Screening for Somatoform Disorders (SOMS 51 ).Psychiatric comorbidities were diagnosed using the German version of the International Neuropsychiatric Interview for Axis I Disorders (MINI 52 ).Comorbid personality disorders were assessed via the German version of the Structured Clinical Interview for DSM-IV Axis II Disorders (SKID II 53 ).Anxiety and Depression were assessed using the Hospital Anxiety and Depression Scale (HADS 54 ).The quality of sleep was measured by means of the Pittsburgh Sleep Quality Index (PSQI 55 ).This includes the variables sleep quality, sleep latency, sleep duration, sleep efficiency, sleep disturbance, the use of sleeping pills and daytime sleepiness.Quality of life was measured using the short form of WHO-Quality of Life 56 .The history of childhood sexual abuse was evaluated via the German version of the Childhood Trauma Questionnaire (CTQ 57 ), which is used to screen for histories of five types of maltreatment during childhood (physical, emotional and sexual abuse as well as a physical and emotional neglect).

(Neuro-)radiological, neurophysiological and gynaecological examination
All subjects received a neurological clinical examination.The neurophysiological examination consisted of electroneurography of peripheral nerves as well as somatosensory evoked potentials (SEP) of the pudendal and tibial nerve.Latencies and amplitudes were measured in SEP.The electroneurography of peripheral nerves included tibial, peroneal, sural, median or ulnar nerve.Nerve conduction velocity (motoric or sensitive), distal motor latency, amplitudes and F-waves were recorded and analysed.
In addition, all subjects received a gynaecological examination.A speculum examination and palpation were performed.Also, a sonographic assessment of the ovary, uterus and endometrium was undertaken.Examination was also performed for varices, free fluid, and possible other abnormalities.The overall impression of the gynaecological examination was assessed by a consultant in gynaecology.
An MRI of the head, pelvis and spinal cord from conus medullaris to sacral spine was performed on a 3T-MR scanner (Magnetom Skyra, Siemens, Germany) to determine possible organic causes (for imaging parameters see supplement 1).Images were interpreted by a consultant of neuroradiology and radiology, respectively.

Data Analysis
All statistical analysis were conducted using SPSS Statistics Version 27 (IBM Corporation, Amonk, NY, USA).Descriptive statistics were calculated and are reported as mean (M) and standard deviation (± /SD).Data were checked for normal distribution using a Kolmogorow-Smirnow test.Analyses were carried out using independent t-tests and Fisher's exact tests for dichotomous variables.As this was the first controlled study to distinguish differences between patients with PGAD and healthy controls regarding the set of clinical variables tested here, an

Sexual history and PGAD symptomatology
According to hypersexual disorder, no group differences were detected (N PGAD = 3, N Controls = 1; p = 0.61).Swelling of the genitals (N PGAD = 17, N Controls = 1) as well as an extraordinary lubrication (N PGAD = 9, N Controls = 2) and spontaneous orgasm (N PGAD = 8, N Controls = 0) showed significantly higher rates in PGAD patients than in controls (p Swelling = 0.00, p Lubrication = 0.04, p Orgasms = 0.00).Significantly more diagnoses of restless legs syndrome were made in the PGAD group (N PGAD = 8, N Controls = 0; p = 0.00).Eight (30.8%) of the PGAD patients were diagnosed with a psychological/psychiatric diagnosis prior to the onset of the PGAD symptomatology.This included depression, anxiety disorders and obsessive-compulsive disorders.None of the subjects showed any abnormalities in the neurological examination (for more details see Table 2).

Symptom severity
On average, actual symptoms were estimated as 3.6 ± 1.9 on a VAS from 0-10.The most severe symptoms in the last 4 weeks averaged 6.8 ± 2.6 and the average symptom severity in the last 4 weeks amounted to an average of 4.9 ± 2.1.

Progression of symptoms
Half of the PGAD patients (13 of 26) stated to have continuous symptoms with slight fluctuations, 7 had continuous symptoms with symptom attacks, 4 had symptom attacks with symptom-free phases in between and 2 of them had symptom attacks with symptoms in between.
Onset of PGAD symptoms 19.2% reported an association of PGAD symptom onset with cessation of a selective serotonin reuptake inhibitor (SSRI) (N = 3) or an anticonvulsant (N = 2).The other subjects could not name an exact initiator for PGAD.
Previous treatment trials with drugs 18 (69.2%)PGAD subjects were taking medication for symptom relief while 8 (30.8%) of the 26 PGAD patients had not yet attempted PGAD medication at the time of the study.14 subjects (77.8%) reported that their medication reduced their symptoms (Fisher's exact test (N = 26), p = 0.00).Selective serotonin noradrenalin reuptake inhibitors (SNRI) and anticonvulsants were most commonly taken.More details on the subjective evaluation of the effect of the particular drugs can be found in Table 4.

Psychological features including psychiatric comorbidities and personality
The PGAD group showed significantly higher rates in sexual dysfunction (N PGAD = 21, N Controls = 1; p = 0.00).Significant group differences were found regarding lack or loss of sexual desire (p = 0.00), sexual aversion (p = 0.00), lack of sexual satisfaction (p = 0.01) and non-organic dyspareunia (p = 0.01).All PGAD patients reported development of sexual dysfunction after the onset of PGAD symptomatology.For more details see Table 5. Regarding sleep, the PSQI revealed decreased sleep quality in PGAD patients (PGAD = 80.8%, Controls = 46.2%,p = 0.02).
Regarding quality of life, the PGAD group reported significantly lower scores in the WHO-QOL-Bref than the control-group.This concerned all subscales (physical health, social relationship, psychological health) except the environmental health domain (t overall (50) = 8.6, p = 0.00).No group differences could be detected on the CTQ subscales.Moreover, neither the PGAD patients nor the controls met the criteria for somatization disorder (for details see Table 5).
The rates of all psychiatric comorbidities in axis I psychiatric disorders were higher in the PGAD group than in the control group (96.2% vs. 34.6%;p = 0.00).In PGAD patients, rates were significantly highest for previous major depression (N PGAD = 22, N Controls = 6; p = 0.00).Also, significantly higher rates for recurrent depression could be detected (N PGAD = 9, N Controls = 0; p = 0.00), but no differences could be detected regarding current depression (N PGAD = 5; N Controls = 0; p = 0.05).There were significantly more PGAD patients with agoraphobia (N PGAD = 8, N Controls = 0; p = 0.00) and generalized anxiety disorder (N PGAD = 6, N Controls = 0; p = 0.02).The rates of diagnoses in depression and anxiety found in the MINI were supported by the psychometric assessment of the Hospital Anxiety and Depression Scale: The scoring for a diagnosis of current depression was not significantly different between groups (N PGAD = 6, N Controls = 1; p = 0.10).Clearly higher scores of anxiety disorders could be detected in the PGAD group (N PGAD = 8, N Controls = 1; p = 0.02).There was a significantly higher rate of lifetime panic disorder in PGAD patients (N PGAD = 9, N Controls = 0; p = 0.00) but not a significant difference between groups in current panic disorder (N PGAD = 4, N Controls = 0; p = 0.11).Social anxiety disorder (N PGAD = 2, N Controls = 0; p = 0.49) occurred without any group differences, as well as obsessive-compulsive disorders (N PGAD = 5, N Controls = 2; p = 0.42).Regarding suicidality, no group differences could be detected (N PGAD = 5, N Controls = 2; p = 0.42).For alcohol use disorder of moderate or low severity no group differences could be detected (N PGAD = 5, N Controls = 3; p = 0.70).None had a high level of alcohol use disorder (see Tables 5 and 6 for all variables).As mentioned above, eight (30.8%) of the PGAD patients were diagnosed with a psychological/psychiatric diagnosis prior to the onset of the PGAD symptomatology.This included depression, anxiety disorders and obsessive-compulsive disorders.In 69.2% psychological comorbidities occurred after the diagnosis of PGAD.
Regarding axis II psychiatric disorders, no group differences could be detected concerning cluster A and cluster C personality disorders (p ClusterA = 0.70; p ClusterC = 0.57).Rates of cluster B personality disorders were significantly higher in the PGAD group compared to the control group (N PGAD = 13, N Controls = 3; p = 0.01).53.8% of the PGAD group fulfilled the DSM-IV criteria for two or more personality disorders, compared with 19.2% of controls (t(50) = -2.2,p = 0.03).For a detailed description of the individual clusters with their personality disorders, see Table 6.

(Neuro-)Radiological, neurophysiological and gynaecological examination
None of the subjects showed any organic abnormality in the brain, except of an accidental finding of a benign tentorium meningioma in one control subject.Overall, 15 (57.7%) subjects from each of the two groups showed abnormalities on MRI in the spinal cord region, without any significant group differences (p = 1.00).The most notable and probably most relevant for PGAD were the fluid-filled nerve root cysts / Tarlov cysts.These occurred particularly at the sacral level of the spine (S1-S4) (except for one control, who also had one thoracic (Th12) and one lumbar (L2) nerve root cyst).There was no significant difference in the occurrence of these cysts between the two groups (N PGAD = 13, N Controls = 9; p = 0.26).Other abnormalities and their frequencies can be found in Table 7. Concerning abnormalities in the MRI of the pelvis, 24 (92.3%)PGAD patients and 23 (88.5%) controls showed abnormalities.No group differences could be detected (p = 1.00).In both groups, the diagnoses were Table 3. PGAD-related characteristics.The sums of the main item radiation differ from the sums of the subitems, because the patients were able to make several statements regarding the individual subitems or had several diagnoses of the individual subitems.Swelling of the genitals, extraordinary lubrication and spontaneous orgasms were mentioned in Table 2 as well as in Table 3, because on the one hand the comparison with the control group was intended to be illustrated, and on the other hand the characterization of the symptomatology was intended to be highlighted by these three criteria.SD standard deviation, PGAD persistent genital arousal disorder.www.nature.com/scientificreports/most commonly ovarian cysts (p = 0.73), followed by venous convolutions (p = 0.78).Pelvic varices were a rare finding without any group differences (N PGAD = 2, N Controls = 0; p = 0.49).For more details see Table 7.
The gynaecological examination showed no differences between the groups either (Fisher's exact test (N = 51), p = 0.11).One PGAD patient did not participate in the gynaecological examination.The abnormalities were mainly confined to the ovaries (for example polycystic ovarian syndrome).www.nature.com/scientificreports/21 PGAD patients and 24 controls participated in the neurophysiological examination.Abnormal examination findings could be identified in 8 PGAD patients and 3 controls (p = 0.08).4 PGAD patients and none of the controls showed prolonged latencies in the SEP of the pudendal nerve (M PGAD = 32.2 ± 6.1 ms; M Controls = 30.5 ± 4.9 ms).No significant differences in the latencies in the group comparison were found (p 1 = 0.35/p 1 = 0.13).The amplitudes of the SEP of the pudendal nerve did also not differ significantly (p = 0.57), see Table 8.However, the occurrence of abnormal latencies (N1, P1) according to clinical assessment differed significantly from each other (Fisher's exact test (N = 45), p = 0.04; t 1 (43) = − 5.192, p 1 = 0.00; t 1 (43) = − 5.587, p 1 = 0.00).Measurements on the tibial, peroneal, median and ulnar nerves were inconspicuous in both groups.

Discussion
To our knowledge this is the first systematic and controlled analysis capturing vegetative, sexual and psychological features, psychiatric comorbidities as well as (neuro-)radiological, neurophysiological and gynaecological examinations in a group of 26 women suffering from PGAD and 26 healthy controls.
According to our data PGAD occurs in all age groups with a possible onset in every stage of life, ranging from late childhood to late adulthood.The average time from symptom onset to diagnosis of PGAD was 5 years, indicating that the disease is still unknown among most physicians.Typically, patients report relatively high and undulating genital arousal levels (~ 7 on a VAS) or continuous arousal with symptom attacks.We found no Table 7. Clinical findings of MRI.Group comparisons between PGAD and control group.PGAD persistent genital arousal disorder.*Denotes significant differences at p < 0.05.p-values were calculated by Fisher's exact test for dichotomous variables (N = 52).The sums of the main item Pelvis and Spinal Cord differ from the sums of the subitems, because the same patient often presented several abnormalities of the individual subitems.On a phenomenological level, PGAD presents as a form of dysesthesia: Almost half of the patients stated that any contact of the genital area, e.g., through tight clothing, was perceived as an unpleasant and also tingling or prickling sensation.These sensations phenomenologically may fit to a small fibre sensory neuropathy of the pudendal nerve 6,12,20,22 , however the current neurophysiological examination did not indicate any pathological findings.Others reported triggering factors like prolonged sitting, lying down and any stimulation in the genital area (vibration, sexual intercourse, masturbation, bus riding, car driving and riding bicycle).These were discussed as a result of injury or entrapment of the pudendal nerve 17,18 .Likewise, the current study did not reveal any reports of traumatization or surgery in the pelvic/genital region.In this respect, the neurophysiological measurements were also inconspicuous except for prolonged SEPs of the pudendal nerve in four of the PGAD patients.Although this did not result in a statistical difference, attention should be paid to this in the future.

MRI
The localization of symptoms included the entire genital and pelvic floor region, with the clitoris being mentioned most frequently (81%), followed by the labia majora and minora (50%) and vaginal opening (46%).This finding is in line with a previous study 22 , showing hypersensitivity in the dermatomes of the dorsal branch of the pudendal nerve and the ilioinguinal nerve.Women in the current study also indicated symptoms of the urethra and the anal region, suggesting that not only the dorsal ramus of the pudendal nerve may be involved but also perineal branches.This would contradict Waldinger's and colleagues' assumption of an isolated neuropathy of the dorsal nerve as cause of PGAD.
Almost 70% of PGAD patients reported radiation to other parts of the body, especially to the legs, the buttocks and the abdominal region.Of note, Tarlov cysts can cause leg, buttock and lower back pain and are also associated with bladder dysfunctions.However, the current study did not show differences in the number of Tarlov cysts between the groups (50% vs. 35%).In single cases PGAD associated sacral cysts were successfully treated by neurosurgical resection 59 or nerve blocks with epidural anaesthesia 18 , although long term outcomes still need to be assessed in these studies.Herniated discs were also rare in women with PGAD and equally frequent in the controls, which also weakens the hypothesis of a radiculopathy.Other abnormalities such as osteochondrosis, sacroiliac joint effusions, ventrolisthesis and spinal stenosis occurred only sporadically and have no meaningful value.
Heat has also been described as a trigger factor, which could be explained by the increased genital blood flow due to the dilation of the vessels by the heat, resulting in a possible higher awareness of genital sensation.Thus, some patients also indicated that cool packs, cold showers, and swimming would help to provide relief.However, more than 20% of the patients also stated that heat would also provide relief, which contradicts the statement above.
Besides modulation of blood circulation and the accompanying chance in awareness by heat, also medication can influence PGAD symptoms.As mentioned above, the onset of PGAD as a discontinuation phenomenon has so far only been reported for drugs with a serotonergic component 2,24,[60][61][62][63][64] .The connection with serotonergic drugs may be explained as follows: When taking an SSRI, serotonin suppresses the production of atrial natriuretic peptide (ANP) in the atrium.If the SSRI is now withdrawn, more ANP is released, which leads to vasodilation and to a return to baseline libido.Consequently, this results in increased genital blood flow and possible newfound awareness of genital sensations 20,65 .Also, a release of the brake of the central nervous system could lead to neuronal hyperexcitability 32 .This discontinuation phenomenon occurred in 5 of the 26 PGAD patients, 3 of whom discontinued an SSRI and 2 of whom reported the phenomenon after discontinuing an anticonvulsant (see also 32 ).Anticonvulsants cause a decrease in neuronal activity, in particular a decrease in neuronal excitation and an increase in neuronal inhibition 66 .Cessation of the anticonvulsant could also result in a rebound effect.Most of the patients treated with medication were taking an SNRI or anticonvulsant.The majority of them also reported symptom relief from medication presumably via the modes of action described above.SSRIs, neuroleptics, non-selective monoamine reuptake inhibitors (NSMRIs), opioids, and benzodiazepines were also taken in exceptional cases.However, the evidence of the efficacy of the drugs is not indicative with such a small number.In summary, the potential benefits of the drugs have not yet been captured widely enough.More accurate data collection on the medication used before the onset of PGAD and on the treatment of PGAD should be considered in future studies.
In summary, a number of trigger and relief factors were reported and the trigger for one may even provide relief for the other (e.g., sexual intercourse, lying down, heat/warmth).Thus, the high variability of trigger and relief factors suggests different causes for PGAD, with both explanations in the direction of peripheral and central neurological deficits.Whereas peripheral stimuli with a central origin could also worsen symptoms.Trigger factors are usually accompanied by the activation of mechanoreceptors (pressure and vibration) as well as psychological factors and menstrual cycle (see below).
Remarkably, more than 30% of the PGAD women compared to none controls reported restless legs symptoms, supporting the hypothesis that PGAD may be a variant of RLS and also of overactive bladder syndrome (OAB).Interestingly both syndromes are discussed in terms of a hyperexcitability of the dopaminergic system 26 .The possible relationship of the syndromes and the associated dopamine deficiency could be one reason for the significantly increased rates of urinary urgency and urinary frequency in PGAD women.Another reason for this finding might be the overlap of bladder and genital function with regard to its spinal representation (S2-S4).Nevertheless, there are barely reports on successful and sustained relief of PGAD symptoms by L-Dopa or dopamine agonists.
With respect to other discussed reasons of PGAD pelvic varices and venous convolutes were found equally seldom in both groups.Waldinger et al. 21highlighted the importance of an age-matched control group especially on the prevalence of pelvic varices.For example, Thorne and Stuckey 67 reported a case with pelvic congestion syndrome.In this case, coil embolization as treatment could lead to a reduction of PGAD symptoms.In fact, www.nature.com/scientificreports/there was one patient in the sample of the iPGAD study who had already benefited from a previous coil embolization, but this did not lead to a complete remission.This, together with the equal distribution of varices and cysts, confirms the suspicion that pelvic varices or pelvic congestion syndrome may be a cause in single cases but not be considered as a general etiological factor.From differential diagnostic levels there was no evidence for elevated levels of somatization disorders as a reason for genital symptoms as found in PGAD.Apart from this, current depressive episode or current panic disorder were equally prevalent in both groups.In contrast, significantly more PGAD patients suffered from recurrent or previous depressive episode and lifetime panic disorders, with most of them starting after the onset of PGAD symptoms (7 of 26 PGAD patients had depression prior to PGAD, but in 18 patients' depression occurred after the onset of PGAD), suggesting that PGAD has a negative impact on the patient's mental health instead of the reverse (depression as a cause of PGAD).The significantly higher incidence of agoraphobia and generalized anxiety disorder in the PGAD group could also result from persistent sexual arousal/agitation as they fear that someone might detect their genital arousal.The patients no longer dare to be around people and large areas, a constant apprehension and tension as well as free-floating fears can be aggravated by the persistent agitation as explained in the fear avoidance model by Jackowich and Pukall 12 .If there is an arousal experience, the patients start to interpret the arousal as alarming and begin to catastrophize.This leads to arousal-related fear and to an avoiding of behaviours related to the arousal.This results in increased hypervigilance to the arousal.The attempt to avoid the hypervigilant arousal eventually leads to negative psychosocial outcomes like depression, anxiety and dysfunctional social interaction.This in turn increases the awareness of the arousal and allows the vicious circle to continue 12,40 .The model is also consistent with the finding that more than 50% of the PGAD patients experienced symptom relief through distraction and symptoms were triggered by stress which leads to elevated levels of vigilance.
PGAD has a tremendous impact on quality of life as indicated by WHO-QOL-BREF measures and also sleep quality was reduced significantly.No differences were found regarding other psychological comorbidities such as alcohol disorders, obsessive-compulsive disorders, social anxiety, eating disorders and PTSD.Particularly noteworthy is the lack of significant difference between the groups regarding suicidality, which has been frequently postulated, e.g., by 10,40,42,43 .The assumption that PGAD arises from trauma like sexual abuse could not be supported by the childhood trauma questionnaire (CTQ) nor the psychiatric history.A questionnaire on sexual trauma after childhood was not explicitly included in the examinations.However, a detailed sexual history was taken, and-according to clinical experience-a possible severe sexual trauma would have been reported.Also, as mentioned above, PTSD was not an issue in both groups.No abnormalities in personality disorders could be identified in the PGAD patients as no differences were found in cluster A and C disorders.However, cluster B disorders occurred significantly more often in PGAD patients, but the individual personality disorders that fall under cluster B did not differ.Overall, the mental constitution of the patients was hardly different from that of the control group.Thus, we assume that PGAD patients are psychologically healthy, except for the mental burden resulting from PGAD.
Since PGAD is a rare disease, a reasonably good number of subjects have already been recruited in the iPGAD study.However, an even larger sample in a design with healthy controls is needed to further corroborate the presented findings, especially regarding the neurophysiological data, and the use of medication.It remained partly unclear to what extent a somatoform autonomic dysfunction (F45.34) could be present in the patients.For this purpose, a group with diagnosed somatoform autonomic dysfunctions should have been included in the comparisons.Also, for more detailed comparisons, with regard to the theory of (functional) nerve lesions, a group of patients should have been included who were treated surgically or who could achieve an improvement through physical therapy.The distinction between PGAD as being a consequence or a cause of mental illness (especially personality disorders and also general anxiety disorder and agoraphobia) remains partially unclear and needs to be examined in more detail.The most likely interpretation is towards a consequence of PGAD.PGAD presents as a heterogeneous syndrome and seems to have multiple triggers.In summary, there was no evidence of a causal relationship to a specific finding as it has been previously discussed.However, further investigations in a larger sample and additional techniques are needed to find out how and where the peripheral or central nervous systems are involved.Regarding clinical implications, health care professionals (in particular general practitioners, psychiatrists, psychotherapists, gynaecologists, urologists and neurologists) should be aware of PGAD symptoms.PGAD is a rare-but possibly highly underreported-and serious disease that can be recognized by already assessing the main symptoms of PGAD as well as triggering and relieving factors.This at least allows symptomatic therapy to be initiated quickly by using combinations of physiotherapy, psychopharmacotherapy, and/or psychotherapy.In addition, psychiatric comorbidities and, where appropriate, quality of life and sleep quality should be taken into account.

Figure 2 .
Figure 2. Recruitment of healthy control group.

Figure 3 .
Figure 3. Localization of PGAD symptoms: Own illustration of a perineum in lithotomy position showing the localization of PGAD symptoms with their frequencies in percent.Multiple answers of each patient were possible.

Table 1 .
Sociodemographic , physical, cognitive, and family factors.M mean, SD standard deviation, df degrees of freedom, PGAD Persistent genital arousal disorder.*Denotessignificantdifferences at p < 0.05.+Indicatescalculated independent t-tests with their t-and p-values and degrees of freedom (df).Single numbers in the statistic-column represent the p-value, calculated by using Fisher's exact test for dichotomous variables (N = 52).t-valuesarein[italic].

Table 2 .
Vegetative, gynaecological and sexual characteristics.M mean, SD standard deviation, df degrees of freedom, PGAD persistent genital arousal disorder.*Denotes significant differences at p < 0.05.
+ Indicates calculated independent t-tests with their t-and p-values and degrees of freedom (df).Single numbers in the statistic-column represent the p-value, calculated by using Fisher's exact test for dichotomous variables (N = 52).t-valuesare in [italic].Vol.:(0123456789)ScientificReports|(2023) 13:22814 | https://doi.org/10.1038/s41598-023-48790-2www.nature.com/scientificreports/buttocks, 4 in the abdominal region and 3 in the back. 2 of each group reported radiation to the arms, breasts and bladder (see Fig.4).Symptom quality 11 patients (42.3%) reported a strong or very strong tingling or prickling sensation.Just as many felt strong or very strong unpleasant sensations when the region was slightly touched for example by clothing or the bedspread.8 PGAD subjects (30.8%) described the symptoms as electrifying.7 patients (26.9%) experienced heat/warmth or cold as well as slight pressure, e.g. with the fingers, as symptomatic.7 (26.9%)subjects felt a hardly or slight burning symptomatology.2 (7.7%) subjects associated the symptomatology with numbness.

Table 5 .
58ychological characteristics and comorbidities.Group comparisons between PGAD and control group.The diagnoses of anxiety and depression were made with a cut off of 11 points or more with the Hospital Anxiety and Depression Scale (HADS)58.Diagnosis of Anxiety: PGAD versus Controls = 30.8%versus 3.8%, p = 0.02*; Diagnosis of Depression: PGAD versus Controls = 23.1% vs. 3.8%, p = 0.10.*Denotes significant differences at p < 0.05.p-values were calculated by using independent t-tests or Fisher's exact test for dichotomous variables (N = 52).The sums of the main item Sexual dysfunction differ from the sums of the subitems, because one patient often showed several diagnoses of the individual subitems.M mean, SD standard deviation, PGAD persistent genital arousal disorder.

Table 6 .
Main categories and findings regarding axis I and axis II (comorbid) disorders.Group comparisons between PGAD and control group.PGAD Persistent Genital Arousal Disorder.*Denotes significant differences at p < 0.05.p-values were calculated by using Fisher's exact test for dichotomous variables (N = 52).The sums of the main items differ from the sums of the subitems, because the same patient often showed several diagnoses of the individual subitems.The international neuropsychiatric interview for Axis I Disorders was used to determine the presence of psychiatric disorders.The Structured Clinical Interview for DSM-IV Axis II Disorders was used to determine comorbid personality disorders.Presence of a disorder was coded 1, absence was coded 0.

Table 8 .
Results of neurophysiology, pudendal nerve.Group comparisons between PGAD and control group.dfdegrees of freedom, PGAD persistent genital arousal disorder.*Denotessignificantdifferences at p < 0.05.The single number in the statistic-column represents the p-value, calculated by using Fisher's exact test for dichotomous variables (N = 45).t-valuesarein[italic].relation to symptoms of hypersexual disorder, however many subjects developed sexual dysfunctions after the onset of PGAD; most commonly lack of sexual desire, sexual aversion, and lack of sexual satisfaction was found.
+ Indicates calculated independent t-tests with their t-and p-values and degrees of freedom (df).